Albumin

  Clinical Significance :-

                         Albumin consists of approximately 60% of the total proteins in the body, the other major part being globulin . It is synthesized in the liver and maintains the osmotic pressure in blood.Albumin also helps in the transporation of drugs, hormones and enzymes. Elevated levels are rarely seen and are usually seen and are usually associated with dehydration. Decrease levels are seen in liver disease . Malnutrition, Kidney disorders,increased fluid loss during extensive burns and decrease absorption in gastro-intestinal diseases. 

Principle :-

             Albumin binds with the dye Bromocresol Green in a buffered medium to form a green coloured complex. The intensity of the colour formed is directly proportional to the amount of protein present in the sample.

 Material required :-

• Clean and dry glassware.                                     
• Laboratory glass Pipettes or  micro Pipettes and tips. 
• Colorimeter.

 Normal Value :- 

                         Albumin (Serum):2.7 - 5.3 g/dl   

                         Globulin       :2.3 - 3.6 g/dl

                         A/G Ratio      :1.0 - 2.3 

Sample :-

              Serum , Heparinized/EDTA Blood. 

Procedure :-

                       Pipette into clean and dry test tubes labeled as Blank ( B ) , Standard ( S ), and Test ( T ) :

Addition           Sequence	B	S	T
Reagent	1ml	1ml	1ml
Standard	-	10 ul	-
Sample	-	-	10ul

 Mix well  and  incubate  at  37 ‘ C for 5 mins. Measure absorbance of the standard ( Abs . S ) and Test ( Abs . T ) against Reagent blank.

 

   Calculation  :-

                                              Abs . T

                     Albumin  ( mg/dl ) =    ..............  * 4

                                              Abs . S   

         

           Globulin in (g/dl)= (Total Proteins) - (Albumin)

                                 (in g/dl)        (in g/dl) 

         

           A/G Ratio =  Albumin in g/dl / Globulin in g/dl

     

Preparation and reagent and stability :-

                                        All Reagent are ready to use and are stable till the expiry date mentioned on the label , when stored at 2' to 8' C.

Quality Control :-

                  For accuracy it is necessary to run known controls with every assay.

Limitation & Precautions :-

 

1. Storage conditions as mentioned on the kit to be adhered.
2. Do not freeze or expose the reagent to high temperature and protect from direct as it may affect the performance of the kit.
3. Use clean glassware free from dust .
4. Re-plug the Glucose standard vial after use.

 General System Parameters:-

          Reaction type           : End point 

          Wave length             : 630 nm ( 623 nm )

          Temperature             : 37'C

          Incubation              : 05 minutes 

          Reagent Volume          : 1.0 ml

          Sample Volume           : 10 ul 

          Standard concentration  : 4 mg/dl

          Zero setting            : Reagent Blank

          Light Path              : 1.0 cm