Sodium ( Mono Method )

 Clinical significance :-

                          Sodium play a key role in your body. It helps maintain normal blood pressure, suports  for the work of your nerves and muscles and regulate your body's fluid balance. if the sodium level are decrease called hyponatremia. If the sodium level are increased called the hypernatremia.

Principle :-         

             Sodium a precipitated  as a triple salt with magnesium and uranyl acetate. The excess of uranyl ions are reacted with ferrocyanide in an acidic medium to develop a brownish colour. The intensity of the colour produced in intesity proportional to the concentration of sodium in the sample.                    

 Material required :-

• Clean and dry glassware.                                     
• Laboratory glass Pipettes or  micro Pipettes and tips. 
• Colorimeter.

 Normal Value :- 

                             135 - 155 (mEq/L)

Sample :-

         Serum or Heparinized Plasma.

Procedure :-

                       Pipette into clean and dry test tubes labeled as Blank ( B ) , Standard ( S ), and Test ( T ) :

Addition           Sequence	B	S	T
Reagent	1ml	1ml	1ml
Standard	-	10 ul	-
Sample	-	-	10ul

 Mix well  and  incubate  at  37 ‘ C for 05  mins. Measure absorbance of the standard ( Abs . S ) and Test ( Abs . T ) against Reagent blank at 630 nm.

 

   Calculation  :-

                                              Abs . T

                    Sodium  ( mEq/L ) =    ..............  * 150

                                              Abs . S   

Preparation and reagent and stability :-

                                        All Reagent are ready to use and are stable till the expiry date mentioned on the label , when stored at 2' to 8' C.

Quality Control :-

                  For accuracy it is necessary to run known controls with every assay.

Limitation & Precautions :-

 

1. Storage conditions as mentioned on the kit to be adhered.
2. Do not freeze or expose the reagent to high temperature and protect from direct as it may affect the performance of the kit.
3. Use clean glassware free from dust .
4. Before the assay bring all reagents to room temperature.

 General System Parameters:-

          Reaction type           : End point 

          Wave length             : 630 nm

          Temperature             : 37'C

          Incubation              : 50 minutes 

          Reagent Volume          : 1.0 ml

          Sample Volume           : 10 ul 

          Standard concentration  : 150 mEq/L

          Zero setting            : Reagent Blank

          Light Path              : 1.0 cm