Potassium ( Mono Method )

 Clinical significance :-

                          Potassium is one of the most important minerals in the body . Potassium is an electrolyte that is vital to cell metabolism. It helps transport nutrient into cell and removes waste product out of cells. It is also important in muscle function helping to transmit messages between nerves and muscles. This test measures the amount of potassium in blood.  If  the potassium level are increased called hyperkalemia. if the potassium level are decrease called hypokalemia.

Principle :-

            Potassium react with sodium tetraphenyl boron in a specially prepared buffer to form of colloidal suspension.  The amount of the turbidity produced is directly proportional to the concentration of potassium in the sample.

 Material required :-

• Clean and dry glassware.                                     
• Laboratory glass Pipettes or  micro Pipettes and tips. 
• Colorimeter.

 Normal Value :- 

                             3.5  - 5.5 mEq/L   

                             

Sample :-

           Serum.

Procedure :-

             Pipette into clean and dry test tubes labeled as Blank ( B ) , Standard ( S ), and Test ( T ) :

Addition           Sequence	B	S	T
Reagent	1ml	1ml	1ml
Standard	-	20 ul	-
Sample	-	-	20ul

 Mix well  and  incubate  at  37 ‘ C for 05  mins. Measure absorbance of the standard ( Abs . S ) and Test ( Abs . T ) against Reagent blank.

 

   Calculation  :-

                                              Abs . T

                    Potasium ( mEq/L ) =    ..............  * 5

                                              Abs . S   

Preparation and reagent and stability :-

                                        All Reagent are ready to use and are stable till the expiry date mentioned on the label , when stored at 2' to 8' C.

Quality Control :-

                  For accuracy it is necessary to run known controls with every assay.

Limitation & Precautions :-

 

1. Storage conditions as mentioned on the kit to be adhered.
2. Do not freeze or expose the reagent to high temperature and protect from direct as it may affect the performance of the kit.
3. Use clean glassware free from dust .
4. Before the assay bring all reagents to room temperature.

 General System Parameters:-

          Reaction type           : End point 

          Wave length             : 630 nm 

          Temperature             : 37'C

          Incubation              : 05 minutes 

          Reagent Volume          : 1.0 ml

          Sample Volume           : 20 ul 

          Standard concentration  : 5.0 mg/dl

          Zero setting            : Reagent Blank

          Light Path              : 1.0 cm