Estimation of Haemoglobin

  Clinical Significance :-

                                                         When hemoglobin is increased from normal range then the condition is known as Polycythemia.

When hemoglobin is decrease from normal range then the condition is known as Anemia.

 Principle :-

                         Hemoglobin is converted into to acid haematin by addition of  N/10 HCL resulting brown colored complex which is compared  comparator box , Acid haematin is insoluble in colloidal suspension in fluid. The intensity of color depends on Pharma on amount of acid haematin formed with depends on amount of hemoglobin present in blood.

 Requirement :-

                                   Sahil's haemometer, hemoglobin tube , hemoglobin pipette , blood sample , distilled water and N/10 HCL

 Specimen :-

                    Capillary tube/ anti coagulated (EDTA) blood.
Normal Range :-

                                       Male - 14 - 18 gm%

                                       Female - 12 - 16 gm%

 Method :-

                       Sahil's method or acid haematin method

 Procedure :-

1.       Taken by 10 HCL in hemoglobin tube upto Mark 20 .
2.       Draw blood up to 0.02 ml mark in hemoglobin pipette avoiding any air bubble in it.
3.       Transfer the blood in the hemoglobin tube containing N/10 HCL. 
4.       Mix well and level for 10 minutes.  The hemoglobin to be changed into acid haematin. 
5.       Diluted with distilled water and mix until the color of the test solution match that of the colored glass of the comparator standard. 
6.       Read the lower level of the fluid in the tube, which express the hemoglobin as a gm %.

    

                                                                        HAEMOMETER